Selection on BrFLC1 Is Related to Intraspecific Diversity of Brassica rapa Vegetables

Flowering time is important for Brassica rapa vegetables because premature bolting before harvest can lower yield and quality. FLOWERING LOCUS C (FLC) acts as a key repressor of flowering. In this study, we identified nonsynonymous mutation at the 58th nucleotide exon1 in BrFLC1 (named Pe1+58 (A/C)) by screening resequencing data 199 B. accessions verified being related to flowering variation. Strong linkage inheritance was detected between locus previously reported splicing site intron 6 (Pi6+1 (G/A)), showing co-occurrence BrFLC1Pe1+58(A) BrFLC1Pi6+1(G), named haplotype H1: AG, or BrFLC1Pe1+58(C) BrFLC1Pi6+1(A), H2: CA. The frequency distribution haplotypes skewed H1 turnip, broccoletto, mizuna, komatsuna, taicai, while it H2 caixin, pak choi, zicaitai, wutacai. frequencies two were comparable Chinese cabbage. This indicated that intraspecific diversification. Further analysis cabbage collection revealed from spring ecotype preferred keep AG almost all summer early purified cabbage, indicating had been strongly selected during genetic improvement cabbages. A significant difference F2 individuals with homologous BrFLC1Pi6+1(G) allele but different BrFLC1Pe1+58 (A/C) alleles, independent effects on time. newly allelic diversity be used breeding resistance vegetables.